Endopeptidase C30 dalam berbagai coronavirus sering disebut 3C like proteinase merupakan famili enzim yang ditemukan dal

Endopeptidase C30 dalam berbagai coronavirus, sering disebut "3C-like proteinase", merupakan famili enzim yang ditemukan dalam poliprotein Coronavirus dan memotong polyprotein di dua situs pembelahan diri. Endopeptidase C30 adalah sisteina protease di bawah klan PA, klasifikasi MEROPS C30.

Peptidase C30, Coronavirus endopeptidase

Identifikasi

Simbol

Peptidase_C30

Pfam

InterPro

PROSITE

MEROPS

SCOP

d1q2wb1

SUPERFAMILY

d1q2wb1

Struktur protein yang tersedia:
Pfam	struktur
PDB	RCSB PDB; PDBe; PDBj
PDBsum	ringkasan struktur

SARS coronavirus main proteinase

Oktamer proteinase utama SARS.

Pengidentifikasi

Nomor EC

3.4.22.69

Basis data

IntEnz

tinjauan IntEnz

BRENDA

entri BRENDA

ExPASy

tinjauan NiceZyme

KEGG

entri KEGG

MetaCyc

jalur metabolik

PRIAM

profil

Struktur PDB

RCSB PDB PDBe PDBsum

Pencarian
PMC	artikel
PubMed	artikel
NCBI	protein

Komisi Enzim mencatat keluarga ini sebagai "proteinase utama SARS coronavirus" (Nomor EC: 3.4.22.69). Nama umum "3C" mengacu pada Enterovirus protease 3C.

Fungsi sunting

Enzim ini mengkatalisasi reaksi kimia:

TSAVLQ-SGFRK-NH2 dan SGVTFQ-!GKFKK adalah dua peptida yang sesuai dengan dua situs pembelahan diri dari proteinase seperti SARS 3C

Enzim ini penting dalam pemrosesan polyprotein SARS coronavirus.

Referensi sunting

Goetz DH, Choe Y, Hansell E, Chen YT, McDowell M, Jonsson CB, Roush WR, McKerrow J, Craik CS (July 2007). "Substrate specificity profiling and identification of a new class of inhibitor for the major protease of the SARS coronavirus". Biochemistry. 46 (30): 8744–52. doi:10.1021/bi0621415. PMID 17605471.
Fan K, Wei P, Feng Q, Chen S, Huang C, Ma L, Lai B, Pei J, Liu Y, Chen J, Lai L (January 2004). "Biosynthesis, purification, and substrate specificity of severe acute respiratory syndrome coronavirus 3C-like proteinase". The Journal of Biological Chemistry. 279 (3): 1637–42. doi:10.1074/jbc.m310875200. PMID 14561748.
Akaji K, Konno H, Onozuka M, Makino A, Saito H, Nosaka K (November 2008). "Evaluation of peptide-aldehyde inhibitors using R188I mutant of SARS 3CL protease as a proteolysis-resistant mutant". Bioorganic & Medicinal Chemistry. 16 (21): 9400–8. doi:10.1016/j.bmc.2008.09.057. PMID 18845442.

[1] Goetz DH, Choe Y, Hansell E, Chen YT, McDowell M, Jonsson CB, Roush WR, McKerrow J, Craik CS (July 2007). "Substrate specificity profiling and identification of a new class of inhibitor for the major protease of the SARS coronavirus". Biochemistry. 46 (30): 8744–52. doi:10.1021/bi0621415. PMID 17605471.

[2] Fan K, Wei P, Feng Q, Chen S, Huang C, Ma L, Lai B, Pei J, Liu Y, Chen J, Lai L (January 2004). "Biosynthesis, purification, and substrate specificity of severe acute respiratory syndrome coronavirus 3C-like proteinase". The Journal of Biological Chemistry. 279 (3): 1637–42. doi:10.1074/jbc.m310875200. PMID 14561748.

[3] Akaji K, Konno H, Onozuka M, Makino A, Saito H, Nosaka K (November 2008). "Evaluation of peptide-aldehyde inhibitors using R188I mutant of SARS 3CL protease as a proteolysis-resistant mutant". Bioorganic & Medicinal Chemistry. 16 (21): 9400–8. doi:10.1016/j.bmc.2008.09.057. PMID 18845442.