Blot Western adalah sebuah metode untuk mendeteksi protein pada sampel jaringan. Imunoblot menggunakan elektroforesis gel untuk memisahkan protein asli atau perubahan oleh jarak polipeptida atau oleh struktur 3-D protein. Protein tersebut dikirim ke membran, di mana mereka dideteksi menggunakan antibodi untuk menargetkan protein.
Referensi sunting
- Renart J, Reiser J, Stark GR. "Transfer of proteins from gels to diazobenzyloxymethyl-paper and detection with antisera: a method for studying antibody specificity and antigen structure", Proc Natl Acad Sci U S A, 1979 Jul;76(7):3116-20. PMID: 91164 abstract 2008-03-23 di Wayback Machine.
- Towbin H, Staehelin T, Gordon J. "Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications" Proc Natl Acad Sci U S A, 1979 Sep;76(9):4350-4. PMID: 388439 abstract 2008-03-17 di Wayback Machine.
- Citation's Classic: Burnette
Pranala luar sunting
- Western blot analysis of sub-cellular fractionated samples using the Odyssey Infrared Imaging System (a protocol) 2010-04-20 di Wayback Machine.
- Western blot protocol including example buffers and reprobing procedure 2007-11-14 di Wayback Machine.
- http://kiatsehat2010.blogspot.co.id/2010/12/test-untuk-aids-elisa-dan-western-blot.html
- http://wanenoor.blogspot.co.id/2012/12/metode-penelitian-dengan-cara-blot.html#.WW7VU9SGPcs
- http://blogku-251294.blogspot.co.id/2015/04/test-elisa-atau-western-blot_23.html
- https://belajarbiokimia.wordpress.com/2014/02/12/western-blot/